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PLoS One. 2014 Feb 11;9(2):e88146. doi: 10.1371/journal.pone.0088146. eCollection 2014.

Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.

Author information

1
Department of Pharmacology and Toxicology, University of Lausanne, Lausanne, Switzerland.
2
INSERM UMR 1064 and Transgenic Rat Facility, University of Nantes, Nantes, France.

Abstract

Transcription Activator-Like Effector Nucleases (TALEN) are potential tools for precise genome engineering of laboratory animals. We report the first targeted genomic integration in the rat using TALENs (Transcription Activator-Like Effector Nucleases) by homology-derived recombination (HDR). We assembled TALENs and designed a linear donor insert targeting a pA476T mutation in the rat Glucocorticoid Receptor (Nr3c1) namely GR(dim), that prevents receptor homodimerization in the mouse. TALEN mRNA and linear double-stranded donor were microinjected into rat one-cell embryos. Overall, we observed targeted genomic modifications in 17% of the offspring, indicating high TALEN cutting efficiency in rat zygotes.

PMID:
24523878
PMCID:
PMC3921256
DOI:
10.1371/journal.pone.0088146
[Indexed for MEDLINE]
Free PMC Article

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