Format

Send to

Choose Destination
J Invest Dermatol. 2014 Jul;134(7):2026-2035. doi: 10.1038/jid.2014.86. Epub 2014 Feb 12.

Panx1 regulates cellular properties of keratinocytes and dermal fibroblasts in skin development and wound healing.

Author information

1
Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada. Electronic address: spenuela@uwo.ca.
2
Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada.
3
Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada; Current address: Department of Medicine (Cardiology), Stanford School of Medicine, Stanford, California 94305, USA.
4
Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada.
5
Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada; Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada.

Abstract

Pannexin1 (Panx1), a channel-forming glycoprotein is expressed in neonatal but not in aged mouse skin. Histological staining of Panx1 knockout (KO) mouse skin revealed a reduction in epidermal and dermal thickness and an increase in hypodermal adipose tissue. Following dorsal skin punch biopsies, mutant mice exhibited a significant delay in wound healing. Scratch wound and proliferation assays revealed that cultured keratinocytes from KO mice were more migratory, whereas dermal fibroblasts were more proliferative compared with controls. In addition, collagen gels populated with fibroblasts from KO mice exhibited significantly reduced contraction, comparable to WT fibroblasts treated with the Panx1 blocker, probenecid. KO fibroblasts did not increase α-smooth muscle actin expression in response to TGF-β, as is the case for differentiating WT myofibroblasts during wound contraction. We conclude that Panx1 controls cellular properties of keratinocytes and dermal fibroblasts during early stages of skin development and modulates wound repair upon injury.

PMID:
24522432
DOI:
10.1038/jid.2014.86
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center