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J Gen Microbiol. 1987 Sep;133(9):2679-87.

Electrophoretic and immunochemical study of the lipopolysaccharides produced by chemostat-grown Escherichia coli O157.

Author information

1
Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario.

Abstract

Two chemically different O-polysaccharides, a low molecular mass form of LPS and core LPS produced by chemostat-grown E. coli O157, were analysed by SDS-PAGE, silver staining and immunoblotting. The reactivities of the different O-polysaccharides with antisera prepared against E. coli O157 grown in batch culture, Salmonella O30 or Brucella abortus were very similar, showing that the O-polysaccharides share at least some antigenic determinants. The reactions of the low molecular mass LPS with the antisera indicated it was semi-rough LPS having one repeat unit of the O-polysaccharide attached to core LPS.

PMID:
2452229
DOI:
10.1099/00221287-133-9-2679
[Indexed for MEDLINE]

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