Format

Send to

Choose Destination
See comment in PubMed Commons below
Phytother Res. 2014 Aug;28(8):1240-5. doi: 10.1002/ptr.5123. Epub 2014 Feb 12.

The isolation of Staphylococcus aureus tea tree oil-reduced susceptibility mutants.

Author information

1
Microbiology Group, Department of Biology, New Mexico State University, Las Cruces, NM, 88003, USA.

Abstract

Tea tree oil (TTO)-reduced susceptibility (TTORS) mutants of two Staphylococcus aureus laboratory strains were isolated utilizing TTO gradient plates. Attempts to isolate TTORS mutants employing agar plates containing single TTO concentrations failed. All TTORS mutants demonstrated a small colony variant (SCV) phenotype and produced cells with a smaller diameter, as determined by scanning electron microscopy. The addition of SCV auxotrophic supplements to media did not lead to an increase in TTORS mutant colony size. Revertants were also isolated from the TTORS mutants following growth in drug-free media, and all revertant strains demonstrated phenotypes similar to their respective parent strains. Transmission electron microscopy revealed that an SH1000 TTORS mutant demonstrated a thinner cell wall and novel septal invaginations compared with parent strain SH1000. In addition, comparative genomic sequencing did not reveal any mutations in an SH1000 TTORS mutant previously linked to well-characterized SCV genotypes. This study demonstrates that TTO can select for a unique SCV phenotype.

KEYWORDS:

Staphylococcus aureus; electron microscopy; genomics; reduced susceptibility; small colony variants; tea tree oil

PMID:
24519735
PMCID:
PMC4125540
DOI:
10.1002/ptr.5123
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley Icon for PubMed Central
    Loading ...
    Support Center