Format

Send to

Choose Destination
Methods Mol Biol. 2014;1131:229-40. doi: 10.1007/978-1-62703-992-5_14.

Cloning of recombinant monoclonal antibodies from hybridomas in a single mammalian expression plasmid.

Author information

1
Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.

Abstract

Antibodies are an integral part of biological and medical research. In addition, immunoglobulins are used in many diagnostic tests and are becoming increasingly important in the therapy of diseases. To express antibodies recombinantly, the immunoglobulin heavy and light chains are usually cloned into two different expression plasmids. Here, we describe a method for recombinant antibody expression from a single plasmid.

PMID:
24515469
DOI:
10.1007/978-1-62703-992-5_14
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center