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Methods Mol Biol. 2014;1131:151-81. doi: 10.1007/978-1-62703-992-5_10.

Yeast surface display for antibody isolation: library construction, library screening, and affinity maturation.

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1
Department of Chemical Engineering, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.

Abstract

Antibodies play key roles as reagents, diagnostics, and therapeutics in numerous biological and biomedical research settings. Although many antibodies are commercially available, oftentimes, specific applications require the development of antibodies with customized properties. Yeast surface display is a robust, versatile, and quantitative method for generating these antibodies and is accessible to single-investigator laboratories. This protocol details the key aspects of yeast surface display library construction and screening.

PMID:
24515465
DOI:
10.1007/978-1-62703-992-5_10
[Indexed for MEDLINE]
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