Format

Send to

Choose Destination
Methods Mol Biol. 2014;1096:85-99. doi: 10.1007/978-1-62703-712-9_7.

Human fecal source identification with real-time quantitative PCR.

Author information

1
National Risk Management Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Cincinnati, OH, USA.

Abstract

Waterborne diseases represent a significant public health risk worldwide and can originate from contact with water contaminated with human fecal material. We describe a real-time quantitative PCR (qPCR) method that targets a genetic marker of the human-associated Bacteroides dorei for identification of human fecal pollution in ambient water samples. The following protocol includes water sample collection, filtration, DNA isolation with a sample processing control, qPCR amplification with an internal amplification control, and quality control data analysis.

PMID:
24515362
DOI:
10.1007/978-1-62703-712-9_7
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center