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J Microbiol Methods. 2013 Oct 24. pii: S0167-7012(13)00322-9. doi: 10.1016/j.mimet.2013.10.007. [Epub ahead of print]

Comparison of Neisseria gonorrhoeae MICs Obtained by Etest and Agar Dilution for Ceftriaxone, Cefpodoxime, Cefixime and Azithromycin.

Author information

1
San Francisco Department of Public Health Laboratory, 101 Grove St. Rm. 419, San Francisco, CA, 94102, USA. Electronic address: severin.gose@sfdph.org.
2
Sexually Transmitted Disease Control Branch, Division of Communicable Disease Control, Center for Infectious Diseases, California Department of Public Health, 850 Marina Bay Parkway, Building P, 2nd floor, Richmond, CA 94804-6403, USA. Electronic address: ckong@alamedahealthsystem.org.
3
San Francisco Department of Public Health Laboratory, 101 Grove St. Rm. 419, San Francisco, CA, 94102, USA. Electronic address: yer.lee@sfdph.org.
4
Sexually Transmitted Disease Control Branch, Division of Communicable Disease Control, Center for Infectious Diseases, California Department of Public Health, 850 Marina Bay Parkway, Building P, 2nd floor, Richmond, CA 94804-6403, USA. Electronic address: msamuel@dhs.ca.gov.
5
Sexually Transmitted Disease Control Branch, Division of Communicable Disease Control, Center for Infectious Diseases, California Department of Public Health, 850 Marina Bay Parkway, Building P, 2nd floor, Richmond, CA 94804-6403, USA. Electronic address: heidi.bauer@cdph.ca.gov.
6
University of Alabama at Birmingham, School of Medicine, Division of Infectious Diseases, THT 220 1720 2nd Ave S., Birmingham, AL 35294-0006, USA. Electronic address: pdixon@uab.edu.
7
Neisseria Reference Laboratory, Department of Global Health and Center for AIDS and STD, University of Washington, 908 Jefferson St., Seattle, WA, 98104, USA. Electronic address: sogeo@u.washington.edu.
8
San Francisco Department of Public Health Laboratory, 101 Grove St. Rm. 419, San Francisco, CA, 94102, USA. Electronic address: john.lei@sfdph.org.
9
San Francisco Department of Public Health Laboratory, 101 Grove St. Rm. 419, San Francisco, CA, 94102, USA. Electronic address: mark.pandori@sfdph.org.

Abstract

We evaluated Neisseria gonorrhoeae Etest minimum inhibitory concentrations (MICs) relative to agar dilution MICs for 664 urethral isolates for ceftriaxone (CRO) and azithromycin (AZM), 351 isolates for cefpodoxime (CPD) and 315 isolates for cefixime (CFM). Etest accurately determined CPD, CFM and AZM MICs, but resulted in higher CRO MICs.

KEYWORDS:

AST; AZM; Azithromycin; CFM; CPD; CRO; ESCs; Etest; MIC; Neisseria gonorrhoeae; agar dilution; antimicrobial susceptibility testing; cefixime; cefpodoxime; ceftriaxone; extended-spectrum cephalosporins; extendeded-spectrum cephalosporins; minimum inhibitory concentration

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