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Biochem Biophys Res Commun. 2014 Mar 7;445(2):352-6. doi: 10.1016/j.bbrc.2014.01.194. Epub 2014 Feb 7.

Phospholipid transfer protein (PLTP) deficiency impaired blood-brain barrier integrity by increasing cerebrovascular oxidative stress.

Author information

1
Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education and Ministry of Public Health, Health Science Center, Peking University, Beijing, China.
2
Center of Medical and Health Analysis, Peking University, Beijing, China.
3
Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing, China.
4
Peking University Third Hospital, Beijing, China.
5
Key Laboratory of Atherosclerosis in Universities of Shandong, Institute of Atherosclerosis, Taishan Medical University, Taian, China.
6
Neuroscience Research Institute and Department of Neurobiology, Key Laboratory for Neuroscience, Ministry of Education and Ministry of Public Health, Health Science Center, Peking University, Beijing, China; Peking University Third Hospital, Beijing, China. Electronic address: dchui@bjmu.edu.cn.

Abstract

Phospholipid transfer protein (PLTP) regulates lipid metabolism and plays an important role in oxidative stress. PLTP is highly expressed in blood-brain barrier (BBB), but the role of PLTP in BBB integrity is not clear. In this study, BBB permeability was detected with in vivo multiphoton imaging and Evans blue assay. We found that PLTP deficient mice exhibited increased BBB permeability, as well as decreased expression of tight junction proteins occludin, zona occludens-1 (ZO-1) and claudin-5 in brain vessels. Cerebrovascular oxidative stress increased in PLTP deficient mice, including increased levels of reactive oxygen species (ROS) and lipid peroxidation marker 4-hydroxy-2-nonenal (HNE) and reduced superoxide dismutase (SOD) activity. Dietary supplementation of antioxidant vitamin E increased BBB integrity and tight junction proteins expression via reducing cerebrovascular oxidative stress. These findings indicated an essential role of PLTP in maintaining BBB integrity, possibly through its ability to transfer vitamin E, and modulate cerebrovascular oxidative stress.

KEYWORDS:

Blood–brain barrier; Oxidative stress; Phospholipid transfer protein; Tight junction

PMID:
24513285
DOI:
10.1016/j.bbrc.2014.01.194
[Indexed for MEDLINE]

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