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Stem Cell Reports. 2014 Jan 14;2(1):26-35. doi: 10.1016/j.stemcr.2013.12.002. eCollection 2014 Jan 14.

Bright/Arid3A acts as a barrier to somatic cell reprogramming through direct regulation of Oct4, Sox2, and Nanog.

Author information

1
Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA.
2
Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, Departments of Cell Biology and Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
3
Department of Developmental Biology and Cancer Research, The Hebrew University Medical School, Jerusalem 91120, Israel.

Abstract

We show here that singular loss of the Bright/Arid3A transcription factor leads to reprograming of mouse embryonic fibroblasts (MEFs) and enhancement of standard four-factor (4F) reprogramming. Bright-deficient MEFs bypass senescence and, under standard embryonic stem cell (ESC) culture conditions, spontaneously form clones that in vitro express pluripotency markers, differentiate to all germ lineages, and in vivo form teratomas and chimeric mice. We demonstrate that BRIGHT binds directly to the promoter/enhancer regions of Oct4, Sox2, and Nanog to contribute to their repression in both MEFs and ESCs. Thus, elimination of the BRIGHT barrier may provide an approach for somatic cell reprogramming.

PMID:
24511468
PMCID:
PMC3916758
DOI:
10.1016/j.stemcr.2013.12.002
[Indexed for MEDLINE]
Free PMC Article
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