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Curr Protoc Stem Cell Biol. 2013 Sep 20;26:Unit 1G.4.. doi: 10.1002/9780470151808.sc01g04s26.

Differentiation of hepatocytes from pluripotent stem cells.

Author information

1
Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin.

Abstract

Differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells into hepatocyte-like cells provides a platform to study the molecular basis of human hepatocyte differentiation, to develop cell culture models of liver disease, and to potentially provide hepatocytes for treatment of end-stage liver disease. Additionally, hepatocyte-like cells generated from human pluripotent stem cells could serve as platforms for drug discovery, determination of pharmaceutical-induced hepatotoxicity, and evaluation of idiosyncratic drug-drug interactions. Here, we describe a step-wise protocol previously developed in our laboratory that facilitates the highly efficient and reproducible differentiation of human pluripotent stem cells into hepatocyte-like cells. Our protocol uses defined culture conditions and closely recapitulates key developmental events that are found to occur during hepatogenesis.

KEYWORDS:

definitive endoderm; hESC; hepatocytes; iPSC; liver development

PMID:
24510789
PMCID:
PMC3920294
DOI:
10.1002/9780470151808.sc01g04s26
[Indexed for MEDLINE]
Free PMC Article
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