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FEBS Lett. 1988 Mar 28;230(1-2):171-5.

Bleomycin resistance conferred by a drug-binding protein.

Author information

1
Laboratoire de Microbiologie et Génétique Appliquées du CNRS, CRBGC, Université Paul Sabatier, Toulouse, France.

Abstract

The protein coded by a bleomycin-resistance gene (ble) cloned from producing actinomycetes was purified from a culture of a recombinant E. coli strain and its action on bleomycin was determined by in vitro assays. The protein binds reversibly in a one to one ratio to bleomycin which can no longer cleave DNA. The bleomycin resistance of cells harboring a ble gene could be accounted for by a sequestering effect of the bleomycin-binding protein.

PMID:
2450783
DOI:
10.1016/0014-5793(88)80665-3
[Indexed for MEDLINE]
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