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Biophys J. 2014 Feb 4;106(3):L13-5. doi: 10.1016/j.bpj.2013.12.007.

On the GFP-based analysis of dynamic concentration profiles.

Author information

1
Mathematical and Statistical Computing Laboratory, Division of Computational Bioscience, Center for Information Technology, National Institutes of Health, Bethesda, Maryland.
2
Department of Chemical and Biological Engineering and Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey. Electronic address: stas@princeton.edu.

Abstract

Studies with GFP-tagged proteins can be used to investigate the dynamics of concentration profiles of regulatory proteins in cells and tissues. Analysis of these experiments must account for the finite rate with which the GFP-tagged proteins mature to the fluorescent state. Toward this end, we present an analytical framework that provides an explicit connection between the apparent kinetics of concentration gradients and the rates of GFP maturation.

PMID:
24507618
PMCID:
PMC3945030
DOI:
10.1016/j.bpj.2013.12.007
[Indexed for MEDLINE]
Free PMC Article
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