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Biophys J. 2014 Feb 4;106(3):639-48. doi: 10.1016/j.bpj.2013.11.4493.

Temporal dynamics of microbial rhodopsin fluorescence reports absolute membrane voltage.

Author information

1
Department of Physics, Harvard University, Cambridge, Massachusetts.
2
Biophysics Program, Harvard University, Cambridge, Massachusetts.
3
Department of Physics, Harvard University, Cambridge, Massachusetts; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts. Electronic address: cohen@chemistry.harvard.edu.

Abstract

Plasma membrane voltage is a fundamentally important property of a living cell; its value is tightly coupled to membrane transport, the dynamics of transmembrane proteins, and to intercellular communication. Accurate measurement of the membrane voltage could elucidate subtle changes in cellular physiology, but existing genetically encoded fluorescent voltage reporters are better at reporting relative changes than absolute numbers. We developed an Archaerhodopsin-based fluorescent voltage sensor whose time-domain response to a stepwise change in illumination encodes the absolute membrane voltage. We validated this sensor in human embryonic kidney cells. Measurements were robust to variation in imaging parameters and in gene expression levels, and reported voltage with an absolute accuracy of 10 mV. With further improvements in membrane trafficking and signal amplitude, time-domain encoding of absolute voltage could be applied to investigate many important and previously intractable bioelectric phenomena.

PMID:
24507604
PMCID:
PMC3945107
DOI:
10.1016/j.bpj.2013.11.4493
[Indexed for MEDLINE]
Free PMC Article

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