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Biochem Biophys Res Commun. 1988 Feb 29;151(1):441-9.

Evidence for a Ca2+ channel within the ryanodine receptor complex from cardiac sarcoplasmic reticulum.

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Department of Biochemistry, School of Medicine, University of North Carolina, Chapel Hill 27599.


The solubilized [3H]ryanodine receptor from cardiac sarcoplasmic reticulum was centrifuged through linear sucrose gradients. A single peak of radioactivity with apparent sedimentation coefficient of approximately 30S specifically comigrated with a high molecular weight protein of apparent relative molecular mass approximately 400,000. Incorporation of the ryanodine receptor into lipid bilayers induced single Ca2+ channel currents with conductance and kinetic behavior almost identical to that of native cardiac Ca2+ release channels. These results suggest that the cardiac ryanodine receptor comprises the Ca2+ release channel involved in excitation-contraction coupling in cardiac muscle.

[Indexed for MEDLINE]

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