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PLoS One. 2014 Feb 5;9(2):e88102. doi: 10.1371/journal.pone.0088102. eCollection 2014.

1H-NMR-based metabolic analysis of human serum reveals novel markers of myocardial energy expenditure in heart failure patients.

Author information

1
Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, China ; Key Laboratory For Organ Failure Research, Ministry of Education of the People's Republic of China, Guangzhou, China.
2
Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, China ; Department of Cardiology, The Third Hospital of Southern Medical University, Guangzhou, China.
3
Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
4
Department of Cardiology, Guangzhou General Hospital of PLA, Guangzhou, China.
5
Key Laboratory For Organ Failure Research, Ministry of Education of the People's Republic of China, Guangzhou, China ; Department of Rheumatology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Abstract

OBJECTIVE:

Elevated myocardial energy expenditure (MEE) is related with reduced left ventricular ejection fraction, and has also been documented as an independent predictor of cardiovascular mortality. However, the serum small-molecule metabolite profiles and pathophysiological mechanisms of elevated MEE in heart failure (HF) are still lacking. Herein, we used 1H-NMR-based metabolomics analysis to screen for potential biomarkers of MEE in HF.

METHODS:

A total of 61 subjects were enrolled, including 46 patients with heart failure and 15 age-matched controls. Venous serum samples were collected from subjects after an 8-hour fast. An INOVA 600 MHz nuclear magnetic resonance spectrometer with Carr-Purcell-Melboom-Gill (CPMG) pulse sequence was employed for the metabolomics analysis and MEE was calculated using colored Doppler echocardiography. Metabolomics data were processed using orthogonal signal correction and regression analysis was performed using the partial least squares method.

RESULTS:

The mean MEE levels of HF patients and controls were 139.61±58.18 cal/min and 61.09±23.54 cal/min, respectively. Serum metabolomics varied with MEE changed, and 3-hydroxybutyrate, acetone and succinate were significantly elevated with the increasing MEE. Importantly, these three metabolites were independent of administration of angiotensin converting enzyme inhibitor, β-receptor blockers, diuretics and statins (P>0.05).

CONCLUSIONS:

These results suggested that in patients with heart failure, MEE elevation was associated with significant changes in serum metabolomics profiles, especially the concentration of 3-hydroxybutyrate, acetone and succinate. These compounds could be used as potential serum biomarkers to study myocardial energy mechanism in HF patients.

PMID:
24505394
PMCID:
PMC3914925
DOI:
10.1371/journal.pone.0088102
[Indexed for MEDLINE]
Free PMC Article

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