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J Parasit Dis. 2014 Mar;38(1):106-10. doi: 10.1007/s12639-012-0203-3. Epub 2012 Nov 11.

Biochemical and molecular characterization of the Cyclophyllidean cestode, Cotugnia cuneata (Meggit, 1924), an endoparasite of domestic pigeons, Columba livia domestica.

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1
Parasitology and Microbiology Research Laboratory, Department of Zoology, The University of Burdwan, Burdwan, 713104 WestBengal India.

Abstract

Analysis of the major biochemical constituents of Cotugnia cuneata revealed that the total protein, carbohydrate, glycogen and lipid contents (as percentage of dry weight) were 25.22 ± 0.93, 32.90 ± 0.30, 21.33 ± 0.99 and 9.94 ± 0.42 respectively. The results showed that the carbohydrate content was the highest followed by protein and lipid contents respectively. Glycogen content was relatively high which showed that carbohydrate was mainly present in the form of glycogen in these cestodes. The A + T and G + C contents were obtained as 49.82 and 50.18 % respectively. The phylogenetic tree showed that C. cuneata branched with its closest cluster comprising of Raillietina tunetensis, Raillietina australis, Fuhrmannetta malakartis and Raillietina sonini with 99 % bootstrap support.

KEYWORDS:

Biochemical characterization; Cotugnia cuneata; Gene sequencing and analysis; Phylogenetic tree

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