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Science. 2014 Feb 7;343(6171):656-661. doi: 10.1126/science.1246135.

A structurally distinct human mycoplasma protein that generically blocks antigen-antibody union.

Author information

1
Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
2
Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
3
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
4
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA.
5
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
6
The Center for Physiological Proteomics, The Scripps Research Institute, La Jolla, CA 92037, USA.
7
Synthetic Biology and Bioenergy Group, J Craig Venter Research Institute, Rockville, MD 20850, USA.
8
Comprehensive Cancer Center, Mayo Clinic Arizona, Scottsdale, AZ 85259, USA.
9
Department of Internal Medicine, Division of Hematology, and Department of Immunology, Mayo Clinic, College of Medicine, Rochester, MN 55905, USA.
10
Departments of Pediatrics and Microbiology & Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
11
Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
#
Contributed equally

Abstract

We report the discovery of a broadly reactive antibody-binding protein (Protein M) from human mycoplasma. The crystal structure of the ectodomain of transmembrane Protein M differs from other known protein structures, as does its mechanism of antibody binding. Protein M binds with high affinity to all types of human and nonhuman immunoglobulin G, predominantly through attachment to the conserved portions of the variable region of the κ and λ light chains. Protein M blocks antibody-antigen union, likely because of its large C-terminal domain extending over the antibody-combining site, blocking entry to large antigens. Similar to the other immunoglobulin-binding proteins such as Protein A, Protein M as well as its orthologs in other Mycoplasma species could become invaluable reagents in the antibody field.

PMID:
24503852
PMCID:
PMC3987992
DOI:
10.1126/science.1246135
[Indexed for MEDLINE]
Free PMC Article

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