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Int Immunopharmacol. 2014 Apr;19(2):214-20. doi: 10.1016/j.intimp.2014.01.020. Epub 2014 Feb 4.

The anti-inflammatory potential of Cortex Phellodendron in vivo and in vitro: down-regulation of NO and iNOS through suppression of NF-κB and MAPK activation.

Author information

1
College of Korean Medicine and Institute of Korean Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
2
College of Pharmacy, Kyung Hee University, Seoul 130-701, Republic of Korea.
3
Formulae Pharmacology Department, School of Oriental Medicine, Gachon University, Seong-Nam, Kyunggi-Do, Republic of Korea.
4
Cancer Preventive Material Development Research Center, College of Korean Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
5
College of Korean Medicine and Institute of Korean Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea. Electronic address: wmyang@khu.ac.kr.

Abstract

Cortex Phellodendri amurensis (CPA), derived from the dried bark of Phellodendron amurense Rupr., is a traditional medicine widely used to treat various inflammation-related diseases. The aim of this study was to investigate the anti-inflammatory activity and molecular mechanism of CPA in vivo and in vitro. Mice were pretreated with CPA (200 mg/kg, p.o.) for three consecutive days; 2h after the last CPA treatment, mice were intraperitoneally injected with lipopolysaccharide (LPS) to induce endotoxemia (35 mg/kg). After treatment, we assessed survival rate, protein levels and cytokine expression. In addition, we confirmed the molecular mechanism of anti-inflammatory effects of CPA in LPS-stimulated macrophage RAW 264.7 cells. The results showed that CPA significantly increased mice survival rates and down-regulated LPS-induced interleukin (IL)-6, IL-1β and macrophage chemo-attractant protein (MCP)-1 in serum. In addition, CPA inhibited inducible nitric oxide synthase (iNOS), activation of nuclear factor (NF)-κB by degradation and phosphorylation of IκBα, and attenuated phosphorylation of mitogen-activated protein kinases (MAPKs; ERK 1/2, p38 and JNK) from mice challenged with LPS. Moreover, in RAW 264.7 cells, CPA dose-dependently down-regulated LPS-stimulated NO, iNOS expression, as well as inflammatory cytokines and protein expression, consistent with the results in vivo. The anti-inflammatory properties of CPA in vitro and in vivo suggest its utility for attenuating inflammation-related diseases.

KEYWORDS:

Cytokine; Inflammation; Lipopolysaccharide (LPS); Phellodendron amurense

PMID:
24503166
DOI:
10.1016/j.intimp.2014.01.020
[Indexed for MEDLINE]

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