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Virology. 2014 Feb;450-451:278-89. doi: 10.1016/j.virol.2013.12.021. Epub 2014 Jan 10.

Subcapsular sinus macrophages limit dissemination of West Nile virus particles after inoculation but are not essential for the development of West Nile virus-specific T cell responses.

Author information

1
Department of Pathology, University of Texas Medical Branch (UTMB), Galveston, TX 77555, USA.
2
Department of Microbiology and Immunology, UTMB, Galveston, TX, USA.
3
Vrije Universiteit, Amsterdam, The Netherlands.
4
Department of Pediatrics, UTMB, 301 University Boulevard, Galveston, TX 77555-0436, USA; Sealy Center for Vaccine Development, UTMB, Galveston, TX 77555-0436, USA.
5
Department of Microbiology and Immunology, UTMB, Galveston, TX, USA; Department of Pediatrics, UTMB, 301 University Boulevard, Galveston, TX 77555-0436, USA; Sealy Center for Vaccine Development, UTMB, Galveston, TX 77555-0436, USA. Electronic address: gnmillig@utmb.edu.

Abstract

Macrophages encounter flaviviruses early after injection by arthropod vectors. Using in vivo imaging of mice inoculated with firefly luciferase-expressing single-cycle flavivirus particles (FLUC-SCFV), we examined the initial dissemination of virus particles in the presence or absence of lymph node (LN)-resident macrophages. Higher luciferase activity, indicating higher SCFV gene expression, was detected in the footpad of macrophage-depleted mice after 24h post infection (hpi). Moreover, FLUC-SCFV particles disseminated to the spleen within 14 hpi in macrophage-depleted, but not control mice. Although macrophages presented SCFV to naïve T cells in vitro, depletion of subcapsular sinus (SCS) macrophages did not alter the magnitude or effector function of the WNV-specific CD8(+) T cell response. Together, these results indicate that SCS macrophages play a role in limiting the dissemination of SCFV early in infection but are not required for the generation of a polyfunctional WNV-specific CD8(+) T cell response in the draining LN.

KEYWORDS:

CD8(+) T cell; RepliVAX; Single-cycle flavivirus; West Nile virus; subcapsular sinus macrophages

PMID:
24503091
PMCID:
PMC3939857
DOI:
10.1016/j.virol.2013.12.021
[Indexed for MEDLINE]
Free PMC Article

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