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Virology. 2014 Feb;450-451:84-97. doi: 10.1016/j.virol.2013.11.027. Epub 2013 Dec 25.

The genome, proteome and phylogenetic analysis of Sinorhizobium meliloti phage ΦM12, the founder of a new group of T4-superfamily phages.

Author information

1
Department of Biological Science, Florida State University, Biology Unit I, 230A, 89 Chieftain Way, Tallahassee, FL 32306-4370, United States.
2
Department of Biological Science, Florida State University, Biology Unit I, 230A, 89 Chieftain Way, Tallahassee, FL 32306-4370, United States; Institute of Molecular Biophysics, Florida State University, 91 Chieftan Way Tallahassee, FL 32306-4380 United States.
3
Department of Biological Science, Florida State University, Biology Unit I, 230A, 89 Chieftain Way, Tallahassee, FL 32306-4370, United States. Electronic address: kmjones@bio.fsu.edu.

Abstract

Phage ΦM12 is an important transducing phage of the nitrogen-fixing rhizobial bacterium Sinorhizobium meliloti. Here we report the genome, phylogenetic analysis, and proteome of ΦM12, the first report of the genome and proteome of a rhizobium-infecting T4-superfamily phage. The structural genes of ΦM12 are most similar to T4-superfamily phages of cyanobacteria. ΦM12 is the first reported T4-superfamily phage to lack genes encoding class I ribonucleotide reductase (RNR) and exonuclease dexA, and to possess a class II coenzyme B12-dependent RNR. ΦM12's novel collection of genes establishes it as the founder of a new group of T4-superfamily phages, fusing features of cyanophages and phages of enteric bacteria.

KEYWORDS:

Alphaproteobacteria; Bacteriophage; Class II ribonucleotide reductase; PhiM12; Proteome; Sinorhizobium meliloti; T4-like phage; gp20; gp23; ΦM12

PMID:
24503070
DOI:
10.1016/j.virol.2013.11.027
[Indexed for MEDLINE]
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