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Curr Biol. 2014 Feb 3;24(3):R107-8. doi: 10.1016/j.cub.2013.12.009.

Generation of infectious virus particles from inducible transgenic genomes.

Author information

1
Department of Neurobiology, Stanford University School of Medicine, Stanford, CA 94305, USA.
2
Department of Neurobiology, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: trc@stanford.edu.

Abstract

Arboviruses like dengue virus, yellow fever virus, and West Nile virus are enveloped particles spread by mosquitoes, infecting millions of humans per year, with neither effective vaccines, nor specific antiviral therapies [1,2]. Previous studies of infection and virus replication utilize either purified virus particles or deficient genomes that do not complete the viral life cycle [1,2]. Here we describe transgenic Drosophila strains expressing trans-complementing genomes (referred to as 'replicons') from the arbovirus Sindbis [2]. We use this binary system to produce, for the first time in any metazoan, infectious virus particles through self-assembly from transgenes. Such cell-type specific particle 'launching' could serve as an attractive alternative for the development of virus-based tools and the study of virus biology in specific tissues.

PMID:
24502780
PMCID:
PMC4110053
DOI:
10.1016/j.cub.2013.12.009
[Indexed for MEDLINE]
Free PMC Article
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