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Mol Biol Cell. 2014 Apr;25(7):1127-36. doi: 10.1091/mbc.E13-08-0477. Epub 2014 Feb 5.

BAF-1 mobility is regulated by environmental stresses.

Author information

1
Department of Genetics, Institute of Life Sciences, Hebrew University of Jerusalem, Givat Ram Jerusalem 91904, Israel Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

Abstract

Barrier to autointegration factor (BAF) is an essential component of the nuclear lamina that binds lamins, LEM-domain proteins, histones, and DNA. Under normal conditions, BAF protein is highly mobile when assayed by fluorescence recovery after photobleaching and fluorescence loss in photobleaching. We report that Caenorhabditis elegans BAF-1 mobility is regulated by caloric restriction, food deprivation, and heat shock. This was not a general response of chromatin-associated proteins, as food deprivation did not affect the mobility of heterochromatin protein HPL-1 or HPL-2. Heat shock also increased the level of BAF-1 Ser-4 phosphorylation. By using missense mutations that affect BAF-1 binding to different partners we find that, overall, the ability of BAF-1 mutants to be immobilized by heat shock in intestinal cells correlated with normal or increased affinity for emerin in vitro. These results show BAF-1 localization and mobility at the nuclear lamina are regulated by stress and unexpectedly reveal BAF-1 immobilization as a specific response to caloric restriction in C. elegans intestinal cells.

PMID:
24501420
PMCID:
PMC3967975
DOI:
10.1091/mbc.E13-08-0477
[Indexed for MEDLINE]
Free PMC Article
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