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PLoS One. 2014 Jan 31;9(1):e88028. doi: 10.1371/journal.pone.0088028. eCollection 2014.

Single and combined effects of deoxynivalenol mycotoxin and a microbial feed additive on lymphocyte DNA damage and oxidative stress in broiler chickens.

Author information

  • 1Department for Farm Animals and Veterinary Public Health, Clinic for Avian, Reptile and Fish Medicine, University of Veterinary Medicine, Vienna, Austria ; Department of Animal Hygiene, Behaviour and Management, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt.
  • 2Department of Animal Hygiene, Behaviour and Management, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt ; Department for Farm Animals and Veterinary Public Health, Institute of Animal Nutrition and Functional Plant Compounds, University of Veterinary Medicine, Vienna, Austria.
  • 3Department of Biomedical Sciences, Institute of Pharmacology and Toxicology, University of Veterinary Medicine, Vienna, Austria.
  • 4Department for Farm Animals and Veterinary Public Health, Clinic for Avian, Reptile and Fish Medicine, University of Veterinary Medicine, Vienna, Austria.
  • 5Department of Animal Hygiene, Behaviour and Management, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt.

Erratum in

  • PLoS One. 2014;9(6):e100907.

Abstract

The immune and intestinal epithelial cells are particularly sensitive to the toxic effects of deoxynivalenol (DON). The aim of this experiment was to study the effects of DON and/or a microbial feed additive on the DNA damage of blood lymphocytes and on the level of thiobarbituric acid reactive substance (TBARS) as an indicator of lipid peroxidation and oxidative stress in broilers. A total of forty 1-d-old broiler chicks were randomly assigned to 1 of 4 dietary treatments (10 birds per group) for 5 wk. The dietary treatments were 1) basal diet; 2) basal diet contaminated with 10 mg DON/kg feed; 3) basal diet contaminated with 10 mg DON/kg feed and supplemented with 2.5 kg/ton of feed of Mycofix Select; 4) basal diet supplemented with Mycofix Select (2.5 kg/ton of feed). At the end of the feeding trial, blood were collected for measuring the level of lymphocyte DNA damage of blood and the TBARS level was measured in plasma, heart, kidney, duodenum and jejunum. The dietary exposure of DON caused a significant increase (P = 0.001) of DNA damage in blood lymphocytes (31.99 ± 0.89%) as indicated in the tail of comet assay. Interestingly addition of Mycofix Select to DON contaminated diet decreased (P = 0.001) the DNA damage (19.82 ± 1.75%) induced by DON. In order to clarify the involvement of lipid peroxidation in the DNA damage of DON, TBARS levels was measured. A significant increase (P = 0.001) in the level of TBARS (23 ± 2 nmol/mg) was observed in the jejunal tissue suggesting that the lipid peroxidation might be involved in the DNA damage. The results indicate that DON is cytotoxic and genotoxic to the chicken intestinal and immune cells and the feed additive have potential ability to prevent DNA damage induced by DON.

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