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PLoS One. 2014 Jan 31;9(1):e87765. doi: 10.1371/journal.pone.0087765. eCollection 2014.

Swimming behavior of Pseudomonas aeruginosa studied by holographic 3D tracking.

Author information

1
Applied Physical Chemistry, Ruprecht-Karls-University Heidelberg, Germany ; Institute for Functional Interfaces, IFG, Karlsruhe Institute of Technology, Karlsruhe, Germany.
2
Applied Physical Chemistry, Ruprecht-Karls-University Heidelberg, Germany ; Institute for Functional Interfaces, IFG, Karlsruhe Institute of Technology, Karlsruhe, Germany ; Analytical Chemistry-Biointerfaces, Ruhr-University Bochum, Germany.
3
Institute for Functional Interfaces, IFG, Karlsruhe Institute of Technology, Karlsruhe, Germany.
4
Applied Physical Chemistry, Ruprecht-Karls-University Heidelberg, Germany.
5
Institute for Functional Interfaces, IFG, Karlsruhe Institute of Technology, Karlsruhe, Germany ; Analytical Chemistry-Biointerfaces, Ruhr-University Bochum, Germany.

Abstract

Holographic 3D tracking was applied to record and analyze the swimming behavior of Pseudomonas aeruginosa. The obtained trajectories allow to qualitatively and quantitatively analyze the free swimming behavior of the bacterium. This can be classified into five distinct swimming patterns. In addition to the previously reported smooth and oscillatory swimming motions, three additional patterns are distinguished. We show that Pseudomonas aeruginosa performs helical movements which were so far only described for larger microorganisms. Occurrence of the swimming patterns was determined and transitions between the patterns were analyzed.

PMID:
24498187
PMCID:
PMC3909247
DOI:
10.1371/journal.pone.0087765
[Indexed for MEDLINE]
Free PMC Article

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