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PLoS Genet. 2014 Jan 30;10(1):e1004126. doi: 10.1371/journal.pgen.1004126. eCollection 2014 Jan.

Single cell genomics: advances and future perspectives.

Author information

1
Single Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom.
2
Single Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom ; Laboratory of Reproductive Genomics, Department of Human Genetics, KU Leuven, Belgium.

Abstract

Advances in whole-genome and whole-transcriptome amplification have permitted the sequencing of the minute amounts of DNA and RNA present in a single cell, offering a window into the extent and nature of genomic and transcriptomic heterogeneity which occurs in both normal development and disease. Single-cell approaches stand poised to revolutionise our capacity to understand the scale of genomic, epigenomic, and transcriptomic diversity that occurs during the lifetime of an individual organism. Here, we review the major technological and biological breakthroughs achieved, describe the remaining challenges to overcome, and provide a glimpse into the promise of recent and future developments.

PMID:
24497842
PMCID:
PMC3907301
DOI:
10.1371/journal.pgen.1004126
[Indexed for MEDLINE]
Free PMC Article

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