Send to

Choose Destination
Elife. 2014;3:e01473. doi: 10.7554/eLife.01473. Epub 2014 Feb 4.

Identification of Redeye, a new sleep-regulating protein whose expression is modulated by sleep amount.

Author information

Howard Hughes Medical Institute, University of Pennsylvania, Philadelphia, United States.


In this study, we report a new protein involved in the homeostatic regulation of sleep in Drosophila. We conducted a forward genetic screen of chemically mutagenized flies to identify short-sleeping mutants and found one, redeye (rye) that shows a severe reduction of sleep length. Cloning of rye reveals that it encodes a nicotinic acetylcholine receptor α subunit required for Drosophila sleep. Levels of RYE oscillate in light-dark cycles and peak at times of daily sleep. Cycling of RYE is independent of a functional circadian clock, but rather depends upon the sleep homeostat, as protein levels are up-regulated in short-sleeping mutants and also in wild type animals following sleep deprivation. We propose that the homeostatic drive to sleep increases levels of RYE, which responds to this drive by promoting sleep. DOI:


Sleepless/Lynx-1; acetylcholine signaling; cycling; sleep

Comment in

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for eLife Sciences Publications, Ltd Icon for PubMed Central
Loading ...
Support Center