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Lupus. 2014 Jun;23(7):635-42. doi: 10.1177/0961203314522337. Epub 2014 Feb 3.

Association between anti-U1 ribonucleoprotein antibodies and inflammatory mediators in cerebrospinal fluid of patients with neuropsychiatric systemic lupus erythematosus.

Author information

1
Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University, Japan.
2
Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University, Japan Department of the Control for Rheumatic Diseases, Graduate School of Medicine, Kyoto University, Japan takfujii@kuhp.kyoto-u.ac.jp.
3
Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University, Japan Department of the Control for Rheumatic Diseases, Graduate School of Medicine, Kyoto University, Japan.

Abstract

OBJECTIVE:

We investigated possible associations between neurotoxic inflammatory mediators (IMs) and anti-U1RNP antibodies (Abs) in cerebrospinal fluid (CSF) of neuropsychiatric systemic lupus erythematosus (NPSLE).

METHODS:

Serum and CSF anti-U1RNP Abs were detected using an RNA-immunoprecipitation assay and CSF anti-U1RNP Ab levels were measured by ELISA. IFN-α, MCP-1 and IL-8 levels in CSF were determined by quantitative multiplex cytokine analysis. IM levels were compared among anti-U1RNP-positive and anti-U1RNP-negative NPSLE as well as other rheumatic disease controls (controls).

RESULTS:

Anti-U1RNP Abs were detected in serum (58%) and in CSF (18%) of 82 NPSLE patients. CSF MCP-1 levels were higher in NPSLE than in controls. CSF IFN-α level was higher in CSF anti-U1RNP Ab-positive than in -negative patients or controls. When limited to serum anti-U1RNP Ab-positive patients, however, levels of all three IMs in CSF were higher in CSF anti-U1RNP Ab-positive than in -negative patients. Anti-U1RNP Ab levels in CSF correlated with CSF MCP-1, but not IFN-α and IL-8 levels.

CONCLUSIONS:

CSF anti-U1RNP Ab positivity is associated with increased level of CSF IFN-α. MCP-1 levels correlated with CSF anti-U1RNP Ab levels, whereas the increased CSF MCP-1 was not specific to CSF anti-U1RNP Ab-positive NPSLE.

KEYWORDS:

Anti-U1RNP antibodies; IFN-α; MCP-1; cerebrospinal fluid; neuropsychiatric systemic lupus erythematosus

PMID:
24493282
DOI:
10.1177/0961203314522337
[Indexed for MEDLINE]
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