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PLoS Biol. 2014 Jan 28;12(1):e1001777. doi: 10.1371/journal.pbio.1001777. eCollection 2014 Jan.

Drosophila Tempura, a novel protein prenyltransferase α subunit, regulates notch signaling via Rab1 and Rab11.

Author information

1
Program in Developmental Biology, Baylor College of Medicine, Houston, Texas, United States of America.
2
Program in Developmental Biology, Baylor College of Medicine, Houston, Texas, United States of America ; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America ; Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital, Houston, Texas, United States of America.
3
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America ; Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas, United States of America.
4
Program in Developmental Biology, Baylor College of Medicine, Houston, Texas, United States of America ; Medical Scientist Training Program, Baylor College of Medicine, Houston, Texas, United States of America.
5
Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, Texas, United States of America.
6
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America.
7
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America ; Department of Neurology, Baylor College of Medicine, Houston, Texas, United States of America.
8
Program in Developmental Biology, Baylor College of Medicine, Houston, Texas, United States of America ; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America ; Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital, Houston, Texas, United States of America ; Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas, United States of America ; Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, Texas, United States of America ; Department of Neuroscience, Baylor College of Medicine, Houston, Texas, United States of America.

Abstract

Vesicular trafficking plays a key role in tuning the activity of Notch signaling. Here, we describe a novel and conserved Rab geranylgeranyltransferase (RabGGT)-α-like subunit that is required for Notch signaling-mediated lateral inhibition and cell fate determination of external sensory organs. This protein is encoded by tempura, and its loss affects the secretion of Scabrous and Delta, two proteins required for proper Notch signaling. We show that Tempura forms a heretofore uncharacterized RabGGT complex that geranylgeranylates Rab1 and Rab11. This geranylgeranylation is required for their proper subcellular localization. A partial dysfunction of Rab1 affects Scabrous and Delta in the secretory pathway. In addition, a partial loss Rab11 affects trafficking of Delta. In summary, Tempura functions as a new geranylgeranyltransferase that regulates the subcellular localization of Rab1 and Rab11, which in turn regulate trafficking of Scabrous and Delta, thereby affecting Notch signaling.

PMID:
24492843
PMCID:
PMC3904817
DOI:
10.1371/journal.pbio.1001777
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

The authors have declared that no competing interests exist.

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