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Sci Rep. 2014 Feb 4;4:3954. doi: 10.1038/srep03954.

Identification of germline transcriptional regulatory elements in Aedes aegypti.

Author information

1
1] Division of Biology, MC 156-29, California Institute of Technology, Pasadena, CA 91125, USA [2].
2
Division of Biology, MC 156-29, California Institute of Technology, Pasadena, CA 91125, USA.

Abstract

The mosquito Aedes aegypti is the principal vector for the yellow fever and dengue viruses, and is also responsible for recent outbreaks of the alphavirus chikungunya. Vector control strategies utilizing engineered gene drive systems are being developed as a means of replacing wild, pathogen transmitting mosquitoes with individuals refractory to disease transmission, or bringing about population suppression. Several of these systems, including Medea, UD(MEL), and site-specific nucleases, which can be used to drive genes into populations or bring about population suppression, utilize transcriptional regulatory elements that drive germline-specific expression. Here we report the identification of multiple regulatory elements able to drive gene expression specifically in the female germline, or in the male and female germline, in the mosquito Aedes aegypti. These elements can also be used as tools with which to probe the roles of specific genes in germline function and in the early embryo, through overexpression or RNA interference.

PMID:
24492376
PMCID:
PMC3912481
DOI:
10.1038/srep03954
[Indexed for MEDLINE]
Free PMC Article
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