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FEBS Lett. 2014 Mar 3;588(5):829-35. doi: 10.1016/j.febslet.2014.01.046. Epub 2014 Feb 1.

End-binding protein 1 (EB1) up-regulation is an early event in colorectal carcinogenesis.

Author information

1
Biomedical Engineering Department, Northwestern University, Evanston, IL 60208, USA. Electronic address: stypula@u.northwestern.edu.
2
Biomedical Engineering Department, Northwestern University, Evanston, IL 60208, USA.
3
Department of Medicine, Boston Medical Center, Boston, MA 02118, USA.
4
Department of Internal Medicine, NorthShore University Health System, Evanston, IL 60201, USA.
5
Department of Medicine, Boston Medical Center, Boston, MA 02118, USA. Electronic address: hkroy@bu.edu.

Abstract

End-binding protein (EB1) is a microtubule protein that binds to the tumor suppressor adenomatous polyposis coli (APC). While EB1 is implicated as a potential oncogene, its role in cancer progression is unknown. Therefore, we analyzed EB1/APC expression at the earliest stages of colorectal carcinogenesis and in the uninvolved mucosa ("field effect") of human and animal tissue. We also performed siRNA-knockdown in colon cancer cell lines. EB1 is up-regulated in early and field carcinogenesis in the colon, and the cellular/nano-architectural effect of EB1 knockdown depended on the genetic context. Thus, dysregulation of EB1 is an important early event in colon carcinogenesis.

KEYWORDS:

Adenomatous polyposis coli; Colon cancer; End-binding protein 1; Field carcinogenesis; Sub-diffractional structure

PMID:
24492008
PMCID:
PMC4103177
DOI:
10.1016/j.febslet.2014.01.046
[Indexed for MEDLINE]
Free PMC Article

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