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Biochem Biophys Res Commun. 2014 Feb 21;444(4):662-9. doi: 10.1016/j.bbrc.2014.01.124. Epub 2014 Feb 1.

Sirt3 controls chromosome alignment by regulating spindle dynamics during mitosis.

Author information

1
Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul 140-742, Republic of Korea.
2
Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul 140-742, Republic of Korea. Electronic address: cyjang@sookmyung.ac.kr.

Abstract

Sirt3, one of mammalian sirtuins is a prominent mitochondrial deacetylase that controls mitochondrial oxidative pathways and the rate of reactive oxygen species. Sirt3 also regulates energy metabolism by deacetylating enzymes involved in the metabolic pathway related with lifespan. We report here a novel function of Sirt3 which was found to be involved in mitosis. Depletion of the Sirt3 protein generated unaligned chromosomes in metaphase which caused mitotic arrest by activating spindle assembly checkpoint (SAC). Furthermore, the shape and the amount of the spindles in Sirt3 depleted cells were abnormal. Microtubule (MT) polymerization also increased in Sirt3 depleted cells, suggesting that Sirt3 is involved in spindle dynamics. However, the level of acetylated tubulin was not increased significantly in Sirt3 depleted cells. The findings collectively suggest that Sirt3 is not a tubulin deacetylase but regulates the attachment of spindle MTs to the kinetochore and the subsequent chromosome alignment by increasing spindle dynamics.

KEYWORDS:

Chromosome alignment; Sirt3; Spindle assembly checkpoint; Spindle dynamics

PMID:
24491532
DOI:
10.1016/j.bbrc.2014.01.124
[Indexed for MEDLINE]
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