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PLoS One. 2014 Jan 29;9(1):e87161. doi: 10.1371/journal.pone.0087161. eCollection 2014.

Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, stimulates hepatic autophagy and lipid clearance.

Author information

1
Program of Cardiovaular and Metabolic Disorders, Duke-NUS Graduate Medical hool, Singapore, Singapore.
2
Department of Anatomy, Yong Loo Lin hool of Medicine, National University of Singapore, Singapore, Singapore.
3
Departments of Chemical Biology, Ernest Mario hool of Pharmacy, Rutgers, The State University of New Jersey, Piataway, New Jersey, United States of America.
4
Program of Cardiovaular and Metabolic Disorders, Duke-NUS Graduate Medical hool, Singapore, Singapore ; Sarah W. Stedman Nutrition and Metabolism Center, Departments of Medicine and Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, United States of America.

Erratum in

  • PLoS One. 2014;9(5):e96884.

Abstract

Epigallocatechin gallate (EGCG) is a major polyphenol in green tea that has been shown to have anti-inflammatory, anti-cancer, anti-steatotic effects on the liver. Autophagy also mediates similar effects; however, it is not currently known whether EGCG can regulate hepatic autophagy. Here, we show that EGCG increases hepatic autophagy by promoting the formation of autophagosomes, increasing lysosomal acidification, and stimulating autophagic flux in hepatic cells and in vivo. EGCG also increases phosphorylation of AMPK, one of the major regulators of autophagy. Importantly, siRNA knockdown of AMPK abrogated autophagy induced by EGCG. Interestingly, we observed lipid droplet within autophagosomes and autolysosomes and increased lipid clearance by EGCG, suggesting it promotes lipid metabolism by increasing autophagy. In mice fed with high-fat/western style diet (HFW; 60% energy as fat, reduced levels of calcium, vitamin D3, choline, folate, and fiber), EGCG treatment reduces hepatosteatosis and concomitantly increases autophagy. In summary, we have used genetic and pharmacological approaches to demonstrate EGCG induction of hepatic autophagy, and this may contribute to its beneficial effects in reducing hepatosteatosis and potentially some other pathological liver conditions.

PMID:
24489859
PMCID:
PMC3906112
DOI:
10.1371/journal.pone.0087161
[Indexed for MEDLINE]
Free PMC Article
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