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Sci Rep. 2014 Feb 3;4:3943. doi: 10.1038/srep03943.

Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system.

Author information

1
1] College of life science, Northeast Forestry University, Harbin, China [2] Department of Biochemistry, College of Animal Sciences, Jilin University, China [3].
2
1] Department of Biochemistry, College of Animal Sciences, Jilin University, China [2].
3
1] College of life science, Northeast Forestry University, Harbin, China [2].
4
College of Veterinary Medicine, Jilin University, China.
5
College of life science, Northeast Forestry University, Harbin, China.
6
Department of Biochemistry, College of Animal Sciences, Jilin University, China.

Abstract

Here, we report a convenient and efficient miRNA inhibition strategy employing the CRISPR system. Using specifically designed gRNAs, miRNA gene has been cut at a single site by Cas9, resulting in knockdown of the miRNA in murine cells. Using a modified CRISPR interference system (CRISPRi), inactive Cas9 can reversibly prevent the expression of both monocistronic miRNAs and polycistronic miRNA clusters. Furthermore, CRISPR/CRISPRi is also capable of suppressing genes in porcine cells.

PMID:
24487629
PMCID:
PMC3909901
DOI:
10.1038/srep03943
[Indexed for MEDLINE]
Free PMC Article

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