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Nucleic Acids Res. 1988 Jan 11;16(1):151-63.

An archaebacterial promoter element for stable RNA genes with homology to the TATA box of higher eukaryotes.

Author information

1
Lehrstuhl für Mikrobiologie, Universität Regensburg, FRG.

Abstract

The RNA polymerase of Methanococcus vannielii, in binary complex with two stable RNA operons, protects from exonuclease digestion the region from 32 bp upstream (-32) to 18 bp downstream (+18) of the transcription start site. Contained within this binding region, centered at -25, is an AT-rich sequence which is highly conserved upstream of 26 other archaebacterial tRNA and rRNA genes. We therefore propose the sequence TTTATAATA as a common element of promoters for stable RNA genes in archaebacteria. Both the similarity in sequence and the location of this conserved octanucleotide suggest homology to the eukaryotic TATA box preceding protein encoding genes transcribed by RNA polymerase B.

PMID:
2448746
PMCID:
PMC334618
DOI:
10.1093/nar/16.1.151
[Indexed for MEDLINE]
Free PMC Article

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