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Gene. 2014 Apr 1;538(2):251-7. doi: 10.1016/j.gene.2014.01.052. Epub 2014 Jan 31.

Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Author information

1
Izmir Institute of Technology, Department of Molecular Biology and Genetics, 35430 Urla, Izmir, Turkey.
2
Westphalian Wilhelms-University Münster, Institute for Biochemistry and Biotechnology of Plants, D-48143 Münster, Germany.
3
Izmir Institute of Technology, Department of Molecular Biology and Genetics, 35430 Urla, Izmir, Turkey. Electronic address: caglarkarakaya@iyte.edu.tr.

Abstract

Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.

KEYWORDS:

Beta maritima; Heavy metal toxicity; Ion homeostasis; Nickel; Transporter protein; Yeast

PMID:
24487090
DOI:
10.1016/j.gene.2014.01.052
[Indexed for MEDLINE]

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