Format

Send to

Choose Destination
See comment in PubMed Commons below
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Feb 15;949-950:79-86. doi: 10.1016/j.jchromb.2014.01.008. Epub 2014 Jan 10.

Determination of 26 veterinary antibiotics residues in water matrices by lyophilization in combination with LC-MS/MS.

Author information

1
National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
2
National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. Electronic address: gale@scau.edu.cn.

Abstract

A sensitive, simple and reliable multi-residue method was developed for the determination of 26 widely used veterinary antibiotics including 6 macrolides, 2 pleuromutilins, 4 tetracyclines, 2 lincosamides, 6 fluoroquinolones and 6 sulfonamides in different water matrices using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Water samples were lyophilized to dryness. Target compounds were separated on Zorbax SB-Aq column (150mm×2.1mm i.d., 3.5μm) and determined by LC-MS/MS operating in positive electrospray ionization mode. Spiked at concentration levels of 0.02, 0.4 and 4μgL(-1), recoveries of all target compounds were over 70% except sulfaquinoxaline (59.0% at 0.02μgL(-1)) with relative standard deviations below 20%. Limits of detection (LOD) and limits of quantification (LOQ) of 26 drugs ranged from 0.1 to 6.5ngL(-1) and from 0.3 to 19ngL(-1), respectively. The developed method was successfully applied to the analysis of 26 antibiotics residues in fish pond water, groundwater, biogas digester water, and lagoon wastewater samples collected from local pig farms.

KEYWORDS:

Antibiotics; Environmental water; Liquid chromatography–tandem mass spectrometry; Lyophilization

PMID:
24487039
DOI:
10.1016/j.jchromb.2014.01.008
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center