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J Biosci Bioeng. 2014 Jul;118(1):98-100. doi: 10.1016/j.jbiosc.2013.12.015. Epub 2014 Jan 31.

Bio-imaging of hydroxyl radicals in plant cells using the fluorescent molecular probe rhodamine B hydrazide, without any pretreatment.

Author information

1
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan. Electronic address: asanom@gly.oups.ac.jp.
2
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
3
Department of Pharmacy, Oita University Hospital, 1-1 Idaigaoka Hasama-machi, Yuhu, Oita 879-5593, Japan.

Abstract

Rhodamine B hydrazide can be used to detect hydroxyl radicals in plant cells. RBH was easily inserted into plant cells without any pretreatment, and specifically reacted with intracellular hydroxyl radicals produced by antimycin A. RBH will be a powerful tool for detecting hydroxyl radicals in plant cells.

KEYWORDS:

Hydroxyl radical; Nicotiana tabacum BY-2; Onion epidermal cell; Reactive oxygen species; Rhodamine B hydrazide

PMID:
24485745
DOI:
10.1016/j.jbiosc.2013.12.015
[Indexed for MEDLINE]
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