Format

Send to

Choose Destination
Methods Mol Biol. 2014;1130:113-121. doi: 10.1007/978-1-62703-989-5_9.

Whole-mount skeletal staining.

Author information

1
University of California, Los Angeles, CA, USA.

Abstract

The first step in almost every investigation of skeletal phenotypes is analysis of whole-mount skeletal preparations. Whole-mount skeletal staining permits evaluation of the shapes and sizes of skeletal elements in their appropriate locations. The technique is thus the major method for detecting changes in skeletal patterning. Because cartilage and bone can be distinguished by differential staining, this technique is also a powerful means to assess the pace of skeletal maturation. This protocol covers staining of the pre- and postnatal mouse skeleton using Alcian blue and Alizarin red to identify cartilage and bone, respectively.

PMID:
24482169
PMCID:
PMC5384832
DOI:
10.1007/978-1-62703-989-5_9
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center