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Methods Enzymol. 2014;537:75-91. doi: 10.1016/B978-0-12-411619-1.00005-7.

Adipose tissue angiogenesis assay.

Author information

1
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
2
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA. Electronic address: silvia.corvera@umassmed.edu.

Abstract

Changes in adipose tissue mass must be accompanied by parallel changes in microcirculation. Investigating the mechanisms that regulate adipose tissue angiogenesis could lead to better understanding of adipose tissue function and reveal new potential therapeutic strategies. Angiogenesis is defined as the formation of new capillaries from existing microvessels. This process can be recapitulated in vitro, by incubation of tissue in extracellular matrix components in the presence of pro-angiogenic factors. Here, we describe a method to study angiogenesis from adipose tissue fragments obtained from mouse and human tissue. This assay can be used to define effects of diverse factors added in vitro, as well as the role of endogenously produced factors on angiogenesis. We also describe approaches to quantify angiogenic potential for the purpose of enabling comparisons between subjects, thus providing information on the role of physiological conditions of the donor on adipose tissue angiogenic potential.

KEYWORDS:

Adipocytes; Angiogenesis; Capillary branches; Endothelial cells; Obesity; Sprouts; Vascularization

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