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Am J Pathol. 2014 Apr;184(4):912-923. doi: 10.1016/j.ajpath.2013.12.002. Epub 2014 Jan 28.

Hydrodynamic transfection for generation of novel mouse models for liver cancer research.

Author information

1
Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California; Liver Center, University of California, San Francisco, California. Electronic address: xin.chen@ucsf.edu.
2
Institute of Pathology, University of Greifswald, Greifswald, Germany. Electronic address: diego.calvisi@uni-greifswald.de.

Abstract

Primary liver cancers, including hepatocellular carcinoma and intrahepatic cholangiocarcinoma, are leading causes of cancer-related death worldwide. Recent large-scale genomic approaches have identified a wide number of genes whose deregulation is associated with hepatocellular carcinoma and intrahepatic cholangiocarcinoma development. Murine models are critical tools to determine the oncogenic potential of these genes. Conventionally, transgenic or knockout mouse models are used for this purpose. However, several limitations apply to the latter models. Herein, we review a novel approach for stable gene expression in mouse hepatocytes by hydrodynamic injection in combination with Sleeping Beauty-mediated somatic integration. This method represents a flexible, reliable, and cost-effective tool to generate preclinical murine models for liver cancer research. Furthermore, it can be used as an in vivo transfection method to study biochemical cross talks among multiple pathways along hepatocarcinogenesis and to test the therapeutic potential of drugs against liver cancer.

PMID:
24480331
PMCID:
PMC3969989
DOI:
10.1016/j.ajpath.2013.12.002
[Indexed for MEDLINE]
Free PMC Article

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