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J Clin Microbiol. 2014 Apr;52(4):1262-5. doi: 10.1128/JCM.00214-14. Epub 2014 Jan 29.

Multiplex high-resolution melting analysis as a diagnostic tool for detection of plasmid-mediated AmpC β-lactamase genes.

Author information

1
Creighton University School of Medicine, Department of Medical Microbiology and Immunology, Center for Research in Anti-Infectives and Biotechnology, Omaha, Nebraska, USA.

Abstract

High-resolution melting (HRM) analysis can be a diagnostic tool to evaluate the presence of resistance genes with the added bonus of discriminating sequence modifications. A real-time, multiplex PCR assay using HRM was designed for the detection of plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 96% and 100%, respectively.

PMID:
24478414
PMCID:
PMC3993522
DOI:
10.1128/JCM.00214-14
[Indexed for MEDLINE]
Free PMC Article

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