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J R Soc Interface. 2014 Jan 29;11(93):20130851. doi: 10.1098/rsif.2013.0851. Print 2014 Apr 6.

Nonlinear light-sheet fluorescence microscopy by photobleaching imprinting.

Author information

1
Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University, , St Louis, MO 63130, USA.

Abstract

We present a nonlinear light-sheet fluorescence microscopy (LSFM) scheme based on photobleaching imprinting. By measuring photobleaching-induced fluorescence decay, our method simultaneously achieves a large imaging field of view and a thin optical section. Furthermore, the scattered-light-induced background is significantly reduced, considerably improving image contrast. Our method is expected to expand the application field of LSFM into the optical quasi-ballistic regime, enabling studies on non-transparent biological samples.

KEYWORDS:

light-sheet fluorescence microscopy; nonlinear microscopy; photobleaching

PMID:
24478278
PMCID:
PMC3928927
DOI:
10.1098/rsif.2013.0851
[Indexed for MEDLINE]
Free PMC Article

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