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PLoS One. 2014 Jan 27;9(1):e87467. doi: 10.1371/journal.pone.0087467. eCollection 2014.

Gene polymorphisms of micrornas in Helicobacter pylori-induced high risk atrophic gastritis and gastric cancer.

Author information

1
Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas, Lithuania ; Department of Gastroenterology, Lithuanian University of Health Sciences, Kaunas, Lithuania.
2
Department of Gastroenterology, Hepatology and Infectious Diseases, Otto von Guericke University, Magdeburg, Germany ; Medical Laboratory for Clinical Chemistry, Microbiology and Infectious Diseases, Department of Molecular Genetics, Magdeburg, Germany.
3
Department of Gastroenterology, Hepatology and Infectious Diseases, Otto von Guericke University, Magdeburg, Germany.
4
Faculty of Medicine, University of Latvia, Riga, Latvia ; Digestive Diseases Center GASTRO, Riga, Latvia ; Riga East University Hospital, Riga, Latvia.
5
Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas, Lithuania.
6
Digestive Diseases Center GASTRO, Riga, Latvia ; Riga East University Hospital, Riga, Latvia.
7
Department of Gastroenterology, Lithuanian University of Health Sciences, Kaunas, Lithuania.

Abstract

BACKGROUND AND AIMS:

MicroRNAs (miRNAs) are known for their function as translational regulators of tumor suppressor or oncogenes. Single nucleotide polymorphisms (SNPs) in miRNAs related genes have been shown to affect the regulatory capacity of miRNAs and were linked with gastric cancer (GC) and premalignant gastric conditions. The purpose of this study was to evaluate potential associations between miRNA-related gene polymorphisms (miR-27a, miR-146a, miR-196a-2, miR-492 and miR-608) and the presence of GC or high risk atrophic gastritis (HRAG) in European population.

METHODS:

Gene polymorphisms were analyzed in 995 subjects (controls: n = 351; GC: n = 363; HRAG: n = 281) of European descent. MiR-27a T>C (rs895819), miR-146a G>C (rs2910164), miR-196a-2 C>T (rs11614913), miR-492 G>C (rs2289030) and miR-608 C>G (rs4919510) SNPs were genotyped by RT-PCR.

RESULTS:

Overall, SNPs of miRNAs were not associated with the presence of GC or HRAG. We observed a tendency for miR-196a-2 CT genotype to be associated with higher risk of GC when compared to CC genotype, however, the difference did not reach the adjusted P-value (odds ratio (OR) - 1.46, 95% confidence interval (CI) 1.03-2.07, P = 0.032). MiR-608 GG genotype was more frequent in GC when compared to controls (OR -2.34, 95% CI 1.08-5.04), but significance remained marginal (P = 0.029). A similar tendency was observed in a recessive model for miR-608, where CC + CG vs GG genotype comparison showed a tendency for increased risk of GC with OR of 2.44 (95% CI 1.14-5.22, P = 0.021). The genotypes and alleles of miR-27a, miR-146a, miR-196a-2, miR-492 and miR-608 SNPs had similar distribution between histological subtypes of GC and were not linked with the presence of diffuse or intestinal-type GC.

CONCLUSIONS:

Gene polymorphisms of miR-27a, miR-146a, miR-196a-2, miR-492, miR-492a and miR-608 were not associated with the presence of HRAG, GC or different histological subtypes of GC in European subjects.

PMID:
24475294
PMCID:
PMC3903675
DOI:
10.1371/journal.pone.0087467
[Indexed for MEDLINE]
Free PMC Article
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