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Viruses. 2014 Jan 27;6(2):448-75. doi: 10.3390/v6020448.

Molecular and biological characterization of a new isolate of guinea pig cytomegalovirus.

Author information

1
Division of Pediatric Infectious Diseases, Department of Pediatrics, University of Minnesota Medical School, Minneapolis, MN 55455, USA. schleiss@umn.edu.
2
Division of Pediatric Infectious Diseases, Department of Pediatrics, University of Minnesota Medical School, Minneapolis, MN 55455, USA. smcallis@umn.edu.
3
Department of Veterinary Population Medicine and Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Saint Paul, MN 55108, USA. armie001@umn.edu.
4
Division of Pediatric Infectious Diseases, Department of Pediatrics, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
5
Division of Pediatric Infectious Diseases, Department of Pediatrics, University of Minnesota Medical School, Minneapolis, MN 55455, USA. zabe043@umn.edu.
6
National Center for Genome Resources (NCGR), Santa Fe, NM 87505, USA. tr@ncgr.org.
7
National Center for Genome Resources (NCGR), Santa Fe, NM 87505, USA. jac@ncgr.org.
8
Division of Pediatric Infectious Diseases, Department of Pediatrics, Virginia Commonwealth University School of Medicine, Richmond, VA 23298, USA. mmcvoy@vcu.edu.

Abstract

Development of a vaccine against congenital infection with human cytomegalovirus is complicated by the issue of re-infection, with subsequent vertical transmission, in women with pre-conception immunity to the virus. The study of experimental therapeutic prevention of re-infection would ideally be undertaken in a small animal model, such as the guinea pig cytomegalovirus (GPCMV) model, prior to human clinical trials. However, the ability to model re-infection in the GPCMV model has been limited by availability of only one strain of virus, the 22122 strain, isolated in 1957. In this report, we describe the isolation of a new GPCMV strain, the CIDMTR strain. This strain demonstrated morphological characteristics of a typical Herpesvirinae by electron microscopy. Illumina and PacBio sequencing demonstrated a genome of 232,778 nt. Novel open reading frames ORFs not found in reference strain 22122 included an additional MHC Class I homolog near the right genome terminus. The CIDMTR strain was capable of dissemination in immune compromised guinea pigs, and was found to be capable of congenital transmission in GPCMV-immune dams previously infected with salivary gland‑adapted strain 22122 virus. The availability of a new GPCMV strain should facilitate study of re-infection in this small animal model.

PMID:
24473341
PMCID:
PMC3939465
DOI:
10.3390/v6020448
[Indexed for MEDLINE]
Free PMC Article
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