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Toxicol Lett. 2014 Apr 21;226(2):140-9. doi: 10.1016/j.toxlet.2014.01.021. Epub 2014 Jan 26.

Genome-wide miRNA-profiling of aflatoxin B1-induced hepatic injury using deep sequencing.

Author information

1
The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and the School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
2
The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and the School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
3
Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
4
The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, and the School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: wshyyl@sina.com.

Abstract

Aflatoxin B1 is a potent carcinogen which can induce** hepatocellular carcinoma (HCC) in mammals. Though microRNAs are known to play important roles in tumorigenesis, the functional complexity of microRNAs in AFB1-induced hepatocellular tumorigenesis has not yet been elucidated. Here, we applied Illumina deep sequencing technology for high-throughput profiling of microRNA in rat liver tissue before and after treatment with aflatoxin B1. Analysis of mature miRNAs from different arms of pre-miRNAs allowed us to identify the predominant form of miRNA. We studied the differential expression profile of miRNAs in two libraries, identifying several cancer-related microRNAs which exhibit abnormal expression. KEGG analysis indicated that predicted target genes of differentially expressed miRNAs are involved in cancer-related pathways. Bioinformatic analysis predicted 16 potential novel miRNAs. Our work provides new insights at the miRNA level into AFB1-induced hepatic injury which may lead to HCC.

KEYWORDS:

Aflatoxin B1; Hepatocellular carcinoma; High-throughput sequencing; MicroRNA

PMID:
24472605
DOI:
10.1016/j.toxlet.2014.01.021
[Indexed for MEDLINE]

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