Format

Send to

Choose Destination
See comment in PubMed Commons below
J Clin Endocrinol Metab. 2014 Apr;99(4):1348-56. doi: 10.1210/jc.2013-1366. Epub 2014 Jan 28.

Placental CYP27B1 and CYP24A1 expression in human placental tissue and their association with maternal and neonatal calcitropic hormones.

Author information

1
Division of Nutritional Sciences (K.O.O., S.L., C.C., T.K., B.V.Y.), Cornell University, Ithaca, New York 14853; The University of Rochester School of Medicine (R.A.Q., E.K.P., E.M.C.), Rochester, New York 14642.

Abstract

CONTEXT:

Placental CYP27B1 may contribute to circulating maternal calcitriol concentrations across gestation, but determinants of CYP27B1 and CYP24A1 expression in term human placental tissue are not well established.

OBJECTIVE:

We hypothesized that higher CYP27B1 protein expression would be associated with increased maternal calcitriol during gestation and that CYP27B1 expression would be impacted by substrate availability.

DESIGN:

This was a prospective, longitudinal study.

SETTING:

The study was completed in an urban, prenatal clinic located in Rochester, New York.

PATIENTS:

The study was undertaken in a cohort of 70 pregnant adolescents (≤18 y of age) and their term neonates.

INTERVENTION:

There was no intervention.

MAIN OUTCOMES:

Protein and mRNA expressions of CYP27B1, CYP24A1, and vitamin D receptor were measured in term placental tissue and related to 25-hydroxyvitamin D [25(OH)D], 1,25-dihydroxyvitamin D, PTH, serum total calcium, IL-6, leptin, and osteoprotegerin measured in maternal serum at midgestation and delivery and in umbilical cord serum at birth.

RESULTS:

Placental CYP27B1 protein expression was significantly positively associated with maternal 25(OH)D at both midgestation (n = 68, P = .009) and delivery (n=67, P = .006). Maternal serum 1,25-dihydroxyvitamin D concentrations at midgestation were positively correlated with term placental CYP27B1 mRNA expression (n = 49, P = .002). Significant positive associations were evident between placental CYP27B1 and CYP24A1 protein expression (P = .001, n = 70). Maternal PTH concentrations at midgestation or delivery did not significantly impact placental protein or transcript level of either enzyme. Variability in placental CYP27B1 protein expression was best captured by a model that included maternal midgestation 25(OH)D concentration, placental vitamin D receptor protein expression, and maternal midgestation IL-6 concentrations (P = .002, n = 60, R(2) = 0.22).

CONCLUSIONS:

Higher maternal 25(OH)D during pregnancy was associated with significantly higher placental protein expression of CYP27B1 at term supportive of a link between substrate availability and placental production of calcitriol.

PMID:
24471562
PMCID:
PMC3973783
DOI:
10.1210/jc.2013-1366
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Silverchair Information Systems Icon for PubMed Central
    Loading ...
    Support Center