Format

Send to

Choose Destination
Nucleic Acids Res. 2014 Apr;42(7):4516-26. doi: 10.1093/nar/gku073. Epub 2014 Jan 26.

Functional signature for the recognition of specific target mRNAs by human Staufen1 protein.

Author information

1
Departamento de Biología Molecular y Celular, Centro Nacional de Biotecnología (CSIC), C/Darwin 3, Campus Cantoblanco, 28049 Madrid, Spain, Ciber de Enfermedades Respiratorias (ISCIII), Mallorca, Spain, Servicio de Genómica Computacional, Centro Nacional de Biotecnología (CSIC), C/Darwin 3, Campus Cantoblanco, 28049 Madrid, Spain and Bioinformática de Sistemas, Centro Nacional de Biotecnología (CSIC), C/Darwin 3, Campus Cantoblanco, 28049 Madrid, Spain.

Abstract

Cellular messenger RNAs (mRNAs) are associated to proteins in the form of ribonucleoprotein particles. The double-stranded RNA-binding (DRB) proteins play important roles in mRNA synthesis, modification, activity and decay. Staufen is a DRB protein involved in the localized translation of specific mRNAs during Drosophila early development. The human Staufen1 (hStau1) forms RNA granules that contain translation regulation proteins as well as cytoskeleton and motor proteins to allow the movement of the granule on microtubules, but the mechanisms of hStau1-RNA recognition are still unclear. Here we used a combination of affinity chromatography, RNAse-protection, deep-sequencing and bioinformatic analyses to identify mRNAs differentially associated to hStau1 or a mutant protein unable to bind RNA and, in this way, defined a collection of mRNAs specifically associated to wt hStau1. A common sequence signature consisting of two opposite-polarity Alu motifs was present in the hStau1-associated mRNAs and was shown to be sufficient for binding to hStau1 and hStau1-dependent stimulation of protein expression. Our results unravel how hStau1 identifies a wide spectrum of cellular target mRNAs to control their localization, expression and fate.

PMID:
24470147
PMCID:
PMC3985646
DOI:
10.1093/nar/gku073
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center