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J Biol Chem. 2014 Mar 14;289(11):7764-75. doi: 10.1074/jbc.M113.544478. Epub 2014 Jan 27.

Cortactin scaffolds Arp2/3 and WAVE2 at the epithelial zonula adherens.

Author information

1
From the Division of Molecular Cell Biology, Institute for Molecular Bioscience, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia.

Abstract

Cadherin junctions arise from the integrated action of cell adhesion, signaling, and the cytoskeleton. At the zonula adherens (ZA), a WAVE2-Arp2/3 actin nucleation apparatus is necessary for junctional tension and integrity. But how this is coordinated with cadherin adhesion is not known. We now identify cortactin as a key scaffold for actin regulation at the ZA, which localizes to the ZA through influences from both E-cadherin and N-WASP. Using cell-free protein expression and fluorescent single molecule coincidence assays, we demonstrate that cortactin binds directly to the cadherin cytoplasmic tail. However, its concentration with cadherin at the apical ZA also requires N-WASP. Cortactin is known to bind Arp2/3 directly (Weed, S. A., Karginov, A. V., Schafer, D. A., Weaver, A. M., Kinley, A. W., Cooper, J. A., and Parsons, J. T. (2000) J. Cell Biol. 151, 29-40). We further show that cortactin can directly bind WAVE2, as well as Arp2/3, and both these interactions are necessary for actin assembly at the ZA. We propose that cortactin serves as a platform that integrates regulators of junctional actin assembly at the ZA.

KEYWORDS:

Actin; Arp2/3; Cell Adhesion; Cell Junctions; Cortactin; Cytoskeleton; E-cadherin; WAVE2

PMID:
24469447
PMCID:
PMC3953287
DOI:
10.1074/jbc.M113.544478
[Indexed for MEDLINE]
Free PMC Article
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