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Shanghai Kou Qiang Yi Xue. 2013 Dec;22(6):613-7.

[Detection of the exogenous gene copy number of the transgenic tomato anti-caries vaccine].

[Article in Chinese]

Author information

1
The Special Key Laboratory of Oral Diseases Research, Higher Education Institution of Guizhou Province,China.E-mail:baiguohui1228@126.com.

Abstract

PURPOSE:

To detect the exogenous gene copy number of the transgenic tomato anti-caries vaccine by using the SYBR Green real-time PCR.

METHODS:

Recombinant plasmid pEAC10 and pEPC10 were used as standard to detect genome samples of exogenous gene pacA-ctxB and pacP-ctxB by SYBR green fluorescent quantitation, then the average value was calculated as gene copy number.

RESULTS:

The copy number of the transgenic tomato carrying pacA-ctxB was 1.3 and the pacP-ctxB was 3.2.

CONCLUSIONS:

The transgenic tomato plants which have high stability are low-copy transgenic plants. Supported by National Natural Science Foundation of China (30160086, 81260164), Science and Technical Fund of Guizhou Province (LKZ[2011]41), Project of Technology Innovation Team in Guizhou Province, Leading Academic Discipline Construction Project in Guizhou Province and Excellent Scientific Research Team Cultivation Project in Zunyi Medical College ([2012]12).

PMID:
24469120
[Indexed for MEDLINE]
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